Sedimentation behavior of hypophyseal growth hormone.
نویسندگان
چکیده
In a previous publication, it was reported that solution of the hypophyseal growth hormone in acetate buffers results in an increment of its intrinsic viscosity with an accompanying increase of molecular weight (1). Later, Smith et al. (2) confirmed these observations in ultracentrifugal studies. The purpose of this present investigation has been to study the sedimentation behavior of the hormone in buffers of various pH values.
منابع مشابه
Ultracentrifugation of hypophyseal growth hormone.
Sedimentation velocity was measured in a Spinco ultracentrifuge’ operating at 165,000 X g. The hormone solutions (0.5 per cent) were prepared with borate buffer of pH 9.7 or saline at pH 9.1. Table I presents a typical run in a borate buffer; the sedimentation velocity constant was calculated by the procedure described by Svedberg and Pedersen (3). It may be noted that the individual values of ...
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ورودعنوان ژورنال:
- The Journal of biological chemistry
دوره 201 2 شماره
صفحات -
تاریخ انتشار 1953